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Tests for reducing and non reducing sugars

ilhaan16

what changes can be made to a pratical to obtain a semi qualitative results

Reply 1

username5443076

I mean you could say you measure the mass of the precipitate formed as well as perform colorimetry

Reply 2

QuentinM

Universities Forum Helper

Original post by ilhaan16

what changes can be made to a pratical to obtain a semi qualitative results

Do you mean for Benedict's test? The test is by its nature semi-quantitative, in that the extent to which it turns red (you may get shades of orange, yellow or green with less sugars present) gives you some indication of amount of sugar. To make it more fully quantitative, you could perform colorimetry with standards of known concentration, then create a formal for converting colorimetry numbers into sugar concentration.

Reply 3

seals2001

Remember to add HCL then Sodium Hydrogencarbonate to test for the NRS, in order to catalyse the breakdown of the sugar into a RS. Also remember to add excess Benedict's solution and heat it at 80 C for around 20 minutes.

You will form a precipitate, and will see colour changes in the solution as this precipitate forms. The great the mass of ppt and the greater the colour change all the way unto red, the more RS / NRS was present.

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Tests for reducing and non reducing sugars

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