tazzy0132
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Ok so I know there’s a whole split on which test but what biological test would you do for amylase? Is it biuret since it’s an enzyme and is a protein or would you do the iodine since it breaks down starch?
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seals2001
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(Original post by tazzy0132)
Ok so I know there’s a whole split on which test but what biological test would you do for amylase? Is it biuret since it’s an enzyme and is a protein or would you do the iodine since it breaks down starch?
Aymlase is a protein. So what d you think ?
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tazzy0132
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(Original post by seals2001)
Aymlase is a protein. So what d you think ?
Biuret surely. I’ve just been thinking simply and surely it’s biuret because it’s a protein and detects peptide bonds so now I feel dumb for overthinking
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macpatgh-Sheldon
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Good morning!

If you use a Biuret test, yes it will be positive but it will be with any other protein, too e.g. haemoglobin OR trypsin OR an antibody, etc so the specificity is v low, agreed? i.e. there will be many false positives, yeah?

Amylase breaks down starch [yes, well done!] so take two samples of starch:-

Add iodine to 1st one -----> turns blue-black, yeah?.

Add amylase first to 2nd one - wait a bit then add iodine again, it will either NOT turn blue-black or less so cos there is v little or none of starch left now, with me?

There's your answer!

M [specialist biology tutor]
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tazzy0132
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(Original post by macpatgh-Sheldon)
Good morning!

If you use a Biuret test, yes it will be positive but it will be with any other protein, too e.g. haemoglobin OR trypsin OR an antibody, etc so the specificity is v low, agreed? i.e. there will be many false positives, yeah?

Amylase breaks down starch [yes, well done!] so take two samples of starch:-

Add iodine to 1st one -----> turns blue-black, yeah?.

Add amylase first to 2nd one - wait a bit then add iodine again, it will either NOT turn blue-black or less so cos there is v little or none of starch left now, with me?

There's your answer!

M [specialist biology tutor]
ohhhh okayy I think I get it!! Thank you so much that had cleared it up so so well!
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