Test for amylase
Watch this thread
Announcements
Page 1 of 1
Skip to page:
tazzy0132
Badges:
8
Rep:
?
You'll earn badges for being active around the site. Rep gems come when your posts are rated by other community members.
#1
Ok so I know there’s a whole split on which test but what biological test would you do for amylase? Is it biuret since it’s an enzyme and is a protein or would you do the iodine since it breaks down starch?
0
reply
seals2001
Badges:
16
Rep:
?
You'll earn badges for being active around the site. Rep gems come when your posts are rated by other community members.
#2
Report
#2
(Original post by tazzy0132)
Ok so I know there’s a whole split on which test but what biological test would you do for amylase? Is it biuret since it’s an enzyme and is a protein or would you do the iodine since it breaks down starch?
Ok so I know there’s a whole split on which test but what biological test would you do for amylase? Is it biuret since it’s an enzyme and is a protein or would you do the iodine since it breaks down starch?
0
reply
tazzy0132
Badges:
8
Rep:
?
You'll earn badges for being active around the site. Rep gems come when your posts are rated by other community members.
#3
(Original post by seals2001)
Aymlase is a protein. So what d you think ?
Aymlase is a protein. So what d you think ?
0
reply
macpatgh-Sheldon
Badges:
20
Rep:
?
You'll earn badges for being active around the site. Rep gems come when your posts are rated by other community members.
#4
Report
#4
Good morning!
If you use a Biuret test, yes it will be positive but it will be with any other protein, too e.g. haemoglobin OR trypsin OR an antibody, etc so the specificity is v low, agreed? i.e. there will be many false positives, yeah?
Amylase breaks down starch [yes, well done!] so take two samples of starch:-
Add iodine to 1st one -----> turns blue-black, yeah?.
Add amylase first to 2nd one - wait a bit then add iodine again, it will either NOT turn blue-black or less so cos there is v little or none of starch left now, with me?
There's your answer!
M [specialist biology tutor]
If you use a Biuret test, yes it will be positive but it will be with any other protein, too e.g. haemoglobin OR trypsin OR an antibody, etc so the specificity is v low, agreed? i.e. there will be many false positives, yeah?
Amylase breaks down starch [yes, well done!] so take two samples of starch:-
Add iodine to 1st one -----> turns blue-black, yeah?.
Add amylase first to 2nd one - wait a bit then add iodine again, it will either NOT turn blue-black or less so cos there is v little or none of starch left now, with me?
There's your answer!
M [specialist biology tutor]
0
reply
tazzy0132
Badges:
8
Rep:
?
You'll earn badges for being active around the site. Rep gems come when your posts are rated by other community members.
#5
(Original post by macpatgh-Sheldon)
Good morning!
If you use a Biuret test, yes it will be positive but it will be with any other protein, too e.g. haemoglobin OR trypsin OR an antibody, etc so the specificity is v low, agreed? i.e. there will be many false positives, yeah?
Amylase breaks down starch [yes, well done!] so take two samples of starch:-
Add iodine to 1st one -----> turns blue-black, yeah?.
Add amylase first to 2nd one - wait a bit then add iodine again, it will either NOT turn blue-black or less so cos there is v little or none of starch left now, with me?
There's your answer!
M [specialist biology tutor]
Good morning!
If you use a Biuret test, yes it will be positive but it will be with any other protein, too e.g. haemoglobin OR trypsin OR an antibody, etc so the specificity is v low, agreed? i.e. there will be many false positives, yeah?
Amylase breaks down starch [yes, well done!] so take two samples of starch:-
Add iodine to 1st one -----> turns blue-black, yeah?.
Add amylase first to 2nd one - wait a bit then add iodine again, it will either NOT turn blue-black or less so cos there is v little or none of starch left now, with me?
There's your answer!
M [specialist biology tutor]
0
reply
X
Page 1 of 1
Skip to page:
Quick Reply
Back
to top
to top