stanleycchou
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Can you advise whether the following methodology to find out the growth rate of Penicillium notatum in various temperature is appropriate or not?

My research question:
To what extend does the temperature will affect the growth rate of Penicillium notatum?

Methodology:
1. Get a small colony Penicillium notatum from the teacher
2. Open the lid of malt agar sample dish
3. Cut a small piece of colony and put it on the malt agar of the sample dish
4. Add a small drop of sterilized water to the small piece of colony inside the sample dish
5. Marked the trial no. of the lid
6. Cover the sample dish with the lid
7. Used tape to fixed the lid
8. Put the sample dish on a white paper with length scale
9. Take photo with the sample dish together with the length scale
10. Put the sample dish upside down
11. Store the sample dish in the incubator at temperature 30 degree celsius for 6 days
12. Repeat step no. 1 to 11, complete remaining 4 trials at this temperature.
13. After 6 days, take out the samples dishes from the incubator
14. Put the sample dish on a white paper with length scale
15. Take photo with the sample dish together with the length scale
16. Import the photos of sample dishes to Logger Pro software, use it to calculate the initial area and final area of of the colony
17. Repeat step no. 1 to 12 with different temperature settings (28, 25, 23 and 4 degree celsius) of the incubator.

The growth rate of Penicillium notatum can be calculated by:
Percentage Growth in area over 6 days = (final area of colony - initial area of colony) / initial area of colony x 100%
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Jpw1097
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(Original post by stanleycchou)
Can you advise whether the following methodology to find out the growth rate of Penicillium notatum in various temperature is appropriate or not?

My research question:
To what extend does the temperature will affect the growth rate of Penicillium notatum?

Methodology:
1. Get a small colony Penicillium notatum from the teacher
2. Open the lid of malt agar sample dish
3. Cut a small piece of colony and put it on the malt agar of the sample dish
4. Add a small drop of sterilized water to the small piece of colony inside the sample dish
5. Marked the trial no. of the lid
6. Cover the sample dish with the lid
7. Used tape to fixed the lid
8. Put the sample dish on a white paper with length scale
9. Take photo with the sample dish together with the length scale
10. Put the sample dish upside down
11. Store the sample dish in the incubator at temperature 30 degree celsius for 6 days
12. Repeat step no. 1 to 11, complete remaining 4 trials at this temperature.
13. After 6 days, take out the samples dishes from the incubator
14. Put the sample dish on a white paper with length scale
15. Take photo with the sample dish together with the length scale
16. Import the photos of sample dishes to Logger Pro software, use it to calculate the initial area and final area of of the colony
17. Repeat step no. 1 to 12 with different temperature settings (28, 25, 23 and 4 degree celsius) of the incubator.

The growth rate of Penicillium notatum can be calculated by:
Percentage Growth in area over 6 days = (final area of colony - initial area of colony) / initial area of colony x 100%
Sounds good to me.
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