Cell centrifugation/fractionation questions.Watch
I am doing some exam questions and need help:
Explain why the homogenate was filtered before spinning at a low speed in the centrifuge (2marks).
My answer is- To remove unbroken cells, but there is more to the answer since it is worth 2 marks can anyone elaborate more?
Explain why the organelles in sediment C (Nucleus) could be seen with a transmission electron microscope but not with an optical microscope?
Thanks anyone who can help me.
2) A transmission electron microscope uses a beam of electrons to create an image of the sample on a monitor. An optical microscope uses visible light and lenses to to magnify the sample.
Basically the transmission microscope is more powerful and produces a clearer image.
In this case, because the organelles found in the nucleus are very small and thin, an transmission electron microscope will be needed to create a more accurate image of the sample, therefore it can be analysed a lot easier/better.
Hope it helps.
(if anyone feels this is incorrect, feel free to edit it)