Allelic and Genotypic Frequencies - GeneticsWatch this thread
Sorry that you have received no help for so long!
If the population in Q has one or more of the following features, then the use of the H-W equation will be invalid:-
a) There is selection pressure, possibly due to interaction between two factors
b) Some members of the population migrate in or out
c) There is non-random mating.
If the Hardy-W equation can be applied, you oc still need some data - given e.g. the incidence of a certain genetically transmitted disease, you just need to substitute your known value in the H-W equation to work out the required allelic frequency.
If you would like me to locate any examples for you, please PM me.
M [specialist biology tutor]