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GM experiment

I need to devise an experiment to determine whether all the leaves on a tomato plant would be able to produce the protein Bt toxin (an insecticide) if it was genetically modified to contain the Bt gene (without harming any insects!)


Any ideas?
Reply 1
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Fluffy
15
jcd
I need to devise an experiment to determine whether all the leaves on a tomato plant would be able to produce the protein Bt toxin (an insecticide) if it was genetically modified to contain the Bt gene (without harming any insects!)


Any ideas?


A Western Blot for the Bt protein. Or a Northern for the DNA.
Reply 2
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jcd
OP
1
Sorry I've only ever heard of a southern blot, could you explain?
Reply 3
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Fluffy
15
jcd
Sorry I've only ever heard of a southern blot, could you explain?


you'll find it on google! But basically for a western, you run an SDS-PAGE gel, elctroblot it onto nitrocellulose paper and then hybridise it with antibody against the BT protein. You visualise using electrochemical luminescence - usually via horse radish peroxidase (an 'ELC kit'). You could also do an ELISA - another anitbody technique.

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