A-level biology required practical 1

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BrightBlueStar11
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for the 1st required practical for A-level biology, do you need to add the same volume of buffer solution and hydrogen peroxide, and then the same volume of catalase?

anyone plz
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BlueChicken
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(Original post by BrightBlueStar11)
for the 1st required practical for A-level biology, do you need to add the same volume of buffer solution and hydrogen peroxide, and then the same volume of catalase?

anyone plz
What exactly is the experiment? As in, are you varying enzyme concentration and measuring oxygen production?
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BrightBlueStar11
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(Original post by BlueChicken)
What exactly is the experiment? As in, are you varying enzyme concentration and measuring oxygen production?
enzyme-controlled reactions - how different temperatures can affect the products being synthesised by enzymes.

They haven't specifically mentioned how many of each solution must be added to the experiment
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BlueChicken
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(Original post by BrightBlueStar11)
enzyme-controlled reactions - how different temperatures can affect the products being synthesised by enzymes.

They haven't specifically mentioned how many of each solution must be added to the experiment
So, if you are looking at effect of temperature, everything else will need to be kept the same. So the amounts used will depend on the equipment (size of test/boiling tubes) you are given to use and the concentrations of solutions, as the reaction will need to produce measurably different amounts of gas at different temperatures. In interests of time, and to not waste resources, you are usually given solutions at appropriate concentrations and told amounts to use (as amounts will depend on concentrations of solutions, pH of buffer etc.).
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BrightBlueStar11
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(Original post by BlueChicken)
So, if you are looking at effect of temperature, everything else will need to be kept the same. So the amounts used will depend on the equipment (size of test/boiling tubes) you are given to use and the concentrations of solutions, as the reaction will need to produce measurably different amounts of gas at different temperatures. In interests of time, and to not waste resources, you are usually given solutions at appropriate concentrations and told amounts to use (as amounts will depend on concentrations of solutions, pH of buffer etc.).
thank u very much
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