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thin layer chromatography

I’m doing A level bio and am confused by what the mobile phase and stationary phase in thin layer chromatography are - can someone explain?
Original post by anon50512
I’m doing A level bio and am confused by what the mobile phase and stationary phase in thin layer chromatography are - can someone explain?


stationary phase - the line and dots you draw to start the experiment.
mobile phase - when the pigments move up the TLC paper (or DNA for electrophoresis)
Original post by anon50512
I’m doing A level bio and am confused by what the mobile phase and stationary phase in thin layer chromatography are - can someone explain?


https://chemguide.co.uk/analysis/chromatography/thinlayer.html
stationary phase = the stuff that doesn't move (e.g. the TLC plate)
mobile phase = the stuff that does move (e.g. the solvent) it moves up the stationary phase, and carries the parts of the mixture that you're analysing
Reply 3
ahh thank you!!!

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