The Student Room Group

Question on standard solutions

1.

In the Proteinuria assay practical you made up a number standard solutions of serum albumin to perform your tests. What volume of a 4% solution of albumin stock solution would be needed to make up 1 mL of 0.2% standard solution of albumin.

Is the answer for this 50 ul (with 0.95mL distilled water)?

1.

In the cholesterol assay practical you made up a number standard solution of cholesterol to construct a standard curve. What volume of a 0.5% cholesterol stock solution would be needed to make up 1 mL of standard that contains 15ug of cholesterol?


im losing it help
(edited 1 month ago)
Original post by modus0perandi

1.

In the Proteinuria assay practical you made up a number standard solutions of serum albumin to perform your tests. What volume of a 4% solution of albumin stock solution would be needed to make up 1 mL of 0.2% standard solution of albumin.

Is the answer for this 50 ul (with 0.95mL distilled water)?

1.

In the cholesterol assay practical you made up a number standard solution of cholesterol to construct a standard curve. What volume of a 0.5% cholesterol stock solution would be needed to make up 1 mL of standard that contains 15ug of cholesterol?


im losing it help

The dilution "factor" is 4 ====> 0.2 = x20
So the volume of solution needed is 1/20 of the final volume
The final volume = 1000ul
So, 1000/20 = 50ul is needed.
To make 1000ul you have to add 950ul distilled water
Original post by charco
The dilution "factor" is 4 ====> 0.2 = x20
So the volume of solution needed is 1/20 of the final volume
The final volume = 1000ul
So, 1000/20 = 50ul is needed.
To make 1000ul you have to add 950ul distilled water

how about the other one? the second question
Original post by modus0perandi

1.

In the Proteinuria assay practical you made up a number standard solutions of serum albumin to perform your tests. What volume of a 4% solution of albumin stock solution would be needed to make up 1 mL of 0.2% standard solution of albumin.

Is the answer for this 50 ul (with 0.95mL distilled water)?

1.

In the cholesterol assay practical you made up a number standard solution of cholesterol to construct a standard curve. What volume of a 0.5% cholesterol stock solution would be needed to make up 1 mL of standard that contains 15ug of cholesterol?


im losing it help

0.5% contains 5g in 1000cm3
= 5mg in 1cm3 = 5000ug per ml
you need 15ug per ml
so you need 15/5000 ml = 3 x 10-3 ml
= 3 mml
Original post by charco
0.5% contains 5g in 1000cm3
= 5mg in 1cm3 = 5000ug per ml
you need 15ug per ml
so you need 15/5000 ml = 3 x 10-3 ml
= 3 mml

thank you

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