Question on standard solutions

12

1.

In the Proteinuria assay practical you made up a number standard solutions of serum albumin to perform your tests. What volume of a 4% solution of albumin stock solution would be needed to make up 1 mL of 0.2% standard solution of albumin.

Is the answer for this 50 ul (with 0.95mL distilled water)?

1.

In the cholesterol assay practical you made up a number standard solution of cholesterol to construct a standard curve. What volume of a 0.5% cholesterol stock solution would be needed to make up 1 mL of standard that contains 15ug of cholesterol?

im losing it help

(edited 1 year ago)

Reply 1

charco

Study Forum Helper

18

Original post by modus0perandi

1.

In the Proteinuria assay practical you made up a number standard solutions of serum albumin to perform your tests. What volume of a 4% solution of albumin stock solution would be needed to make up 1 mL of 0.2% standard solution of albumin.

Is the answer for this 50 ul (with 0.95mL distilled water)?

1.

In the cholesterol assay practical you made up a number standard solution of cholesterol to construct a standard curve. What volume of a 0.5% cholesterol stock solution would be needed to make up 1 mL of standard that contains 15ug of cholesterol?

im losing it help

The dilution "factor" is 4 ====> 0.2 = x20
So the volume of solution needed is 1/20 of the final volume
The final volume = 1000ul
So, 1000/20 = 50ul is needed.
To make 1000ul you have to add 950ul distilled water

Reply 2

modus0perandi

OP

12

Original post by charco

The dilution "factor" is 4 ====> 0.2 = x20
So the volume of solution needed is 1/20 of the final volume
The final volume = 1000ul
So, 1000/20 = 50ul is needed.
To make 1000ul you have to add 950ul distilled water

how about the other one? the second question

Reply 3

charco

Study Forum Helper

18

Original post by modus0perandi

1.

In the Proteinuria assay practical you made up a number standard solutions of serum albumin to perform your tests. What volume of a 4% solution of albumin stock solution would be needed to make up 1 mL of 0.2% standard solution of albumin.

Is the answer for this 50 ul (with 0.95mL distilled water)?

1.

In the cholesterol assay practical you made up a number standard solution of cholesterol to construct a standard curve. What volume of a 0.5% cholesterol stock solution would be needed to make up 1 mL of standard that contains 15ug of cholesterol?

im losing it help

0.5% contains 5g in 1000cm³
= 5mg in 1cm³ = 5000ug per ml
you need 15ug per ml
so you need 15/5000 ml = 3 x 10^-3 ml
= 3 mml

Reply 4

modus0perandi

OP

12

Original post by charco

0.5% contains 5g in 1000cm³
= 5mg in 1cm³ = 5000ug per ml
you need 15ug per ml
so you need 15/5000 ml = 3 x 10^-3 ml
= 3 mml

thank you

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