colorimeter

for my bio c/wk i have to use a colorimeter.......i'm going to be using anthrocyanin in it.........so which filter in the colorimeter do i use ???and has the wavelength at which anthrocyanin absorbs light got anything to do with the colorimeter???? :frown:

Reply 1

Saf!

anyone!??? :frown:

Reply 2

samsonmoley

im really not sure if this will be helpful but here are some sites i used whan i was doing some work with a colorimeter - i cant help exactly with your question cus im to dumb and havent sdone the experiment and i dont no what type of colorimeter your using but here you go

http://humboldt.edu/~scimus/Instruments/Color-Myers/Color.html
http://www.lamotte.com/pages/common/pdf/manuals/Stc-1.pdf

also i just saw this bit on this page
The filters are used to isolate a part of the visible light spectrum that is absorbed maximally by the sample. Different colourimeters use different sets of filters but typical wavelengths passed are red filter: 630-750nm, green filter: 510-570nm and blue filter: 360-480nm. Although you will normally be told which filter to use you should consider and understand the reason for this choice.

this is the page it came from
http://www.liv.ac.uk/~agmclen/Medpracs/practical_3/Appendix_3.html

ok well hope it all goes fab - tell me if it does help then ill tell the world
Zoe :cool:

Reply 3

oxymoron

You want to use a filter which lets light through that is absorbed by the sample. For example, if the compound you are measuring is red, then it absorbs blue light so you should use a blue filter.

In other words use the complementary colour to the compound you are measuring.

If you are told the wavelength it absorbs at, then use a filter that lets that wavelength of light through.