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Saf!
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#1
Report Thread starter 15 years ago
#1
for my bio c/wk i have to use a colorimeter.......i'm going to be using anthrocyanin in it.........so which filter in the colorimeter do i use ???and has the wavelength at which anthrocyanin absorbs light got anything to do with the colorimeter????
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Saf!
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#2
Report Thread starter 15 years ago
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anyone!???
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samsonmoley
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#3
Report 15 years ago
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:hello: im really not sure if this will be helpful but here are some sites i used whan i was doing some work with a colorimeter - i cant help exactly with your question cus im to dumb and havent sdone the experiment and i dont no what type of colorimeter your using but here you go

http://humboldt.edu/~scimus/Instrume...ers/Color.html
http://www.lamotte.com/pages/common/...uals/Stc-1.pdf

also i just saw this bit on this page
The filters are used to isolate a part of the visible light spectrum that is absorbed maximally by the sample. Different colourimeters use different sets of filters but typical wavelengths passed are red filter: 630-750nm, green filter: 510-570nm and blue filter: 360-480nm. Although you will normally be told which filter to use you should consider and understand the reason for this choice.

this is the page it came from
http://www.liv.ac.uk/~agmclen/Medpra...ppendix_3.html

ok well hope it all goes fab - tell me if it does help then ill tell the world
Zoe :cool:
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oxymoron
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#4
Report 15 years ago
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You want to use a filter which lets light through that is absorbed by the sample. For example, if the compound you are measuring is red, then it absorbs blue light so you should use a blue filter.

In other words use the complementary colour to the compound you are measuring.

If you are told the wavelength it absorbs at, then use a filter that lets that wavelength of light through.
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Twiglet
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#5
Report 15 years ago
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its called anthocyanin by the way
i used a blue filter and wavelengh was 470 nm
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