Mass Spectrometry MALDI-TOF
Any help would be much appreciated!
Acetylation of the excitatory response regulator CheY by acetyl CoA synthesise is involved in chemotaxis in E. coli.
Acetylation of CheY in vitro using sodium acetate or acetyl CoA gave an additional 6 peaks in the MALDI-TOF spectrum at m/z 14048, 14100, 14177, 14216 and 14476. For each peak account for its mass. Note that the parent peak was observed at m/z 13969.
I know that the addition of an acetyl group is adding roughly 42 Da each time. But other than just making up the difference between the new peaks and the parent with lots of 42s, I'm a bit stuck.
Acetylation of the excitatory response regulator CheY by acetyl CoA synthesise is involved in chemotaxis in E. coli.
Acetylation of CheY in vitro using sodium acetate or acetyl CoA gave an additional 6 peaks in the MALDI-TOF spectrum at m/z 14048, 14100, 14177, 14216 and 14476. For each peak account for its mass. Note that the parent peak was observed at m/z 13969.
I know that the addition of an acetyl group is adding roughly 42 Da each time. But other than just making up the difference between the new peaks and the parent with lots of 42s, I'm a bit stuck.
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