Protein folding multiple choice

In the original Anfinsen protein refolding experiment, ribonuclease was used. Ribonuclease has 8 cysteine residues that form 4 disulfide bridges. The experiment was repeated using protein X with 11 cysteine residues, giving 5 disulfide bridges and one unpartnered cysteine residue. How much activity would scrambled disulfide protein X have? (Hint: scrambled ribonuclease has ~1% activity)
A. 0.026%
B. 0.053%
C. 0.106%
D. 0.212%

Please help I have no idea how to do this

Reply 1

Kallisto

Entertainment Forum Helper, Life & Style Forum Helper

I have no idea how to calculate it too. I tag some members who might be able to help you.

@DerDracologe
@RDKGames
@Lemur14

Reply 2

DerDracologe

Study Forum Helper

Sorry, no idea. What is this for?

Reply 3

Kallisto

Entertainment Forum Helper, Life & Style Forum Helper

Original post

by DerDracologe

Sorry, no idea. What is this for?

It is about the numbers of scrambled disulfides and thus the extent of denaturation in a protein, I guess. But you tell me. Is there a way for calculation anyway? if so, I have really no clue.

@emm4nuella
@flowersinmyhair

Maybe they know it better.

(edited 11 months ago)

Reply 4

flowersinmyhair

Volunteer Section Leader, Clearing and Applications Advisor

Original post

by Kallisto

It is about the numbers of scrambled disulfides and thus the extent of denaturation in a protein, I guess. But you tell me. Is there a way for calculation anyway? if so, I have really no clue.
@emm4nuella
@flowersinmyhair
Maybe they know it better.

I don't know, very sorry :frown:

Reply 5

DerDracologe

Study Forum Helper

Original post

by Kallisto

It is about the numbers of scrambled disulfides and thus the extent of denaturation in a protein, I guess. But you tell me. Is there a way for calculation anyway? if so, I have really no clue.
@emm4nuella
@flowersinmyhair
Maybe they know it better.

This isnt covered (to my knowledge) in the enzymes content ive done at a level so i was wondering whether it’s beyond a level. Ive got no idea how to even start doing something like that- most of my maths questions in bio are converting units 😅

Reply 6

Kallisto

Entertainment Forum Helper, Life & Style Forum Helper

Original post

by DerDracologe

Me too. That is beyond my knowledge too, I had taken biology and chemistry at A levels. I guess it is at least undergraduate.

@Onionkid1717 what is the level of this question?

Reply 7

emm4nuella

Original post

by Kallisto

It is about the numbers of scrambled disulfides and thus the extent of denaturation in a protein, I guess. But you tell me. Is there a way for calculation anyway? if so, I have really no clue.
@emm4nuella
@flowersinmyhair
Maybe they know it better.