Genetic problem

You have isolated a plasmid from E. coli
and wish to begin your analysis of it by
making a restriction map. Using three
restriction enzymes, B (BamHI), E(EcoRI), N
(NotI), you perform six different digestions:
single digests using each enzyme alone and
double digests using each combination of two
enzymes. Agarose gel electrophoresis of the
resulting fragments produces the results
shown here. Draw a final restriction map of
the plasmid