1 cut (healthy) shoot under water (to stop air entering xylem vessels);
2 cut shoot at a slant (to increase surface area);
3 check apparatus is full of water / is air bubble free / no air locks;
4 insert shoot into apparatus under water / AW;
5 remove potometer from water and ensure , airtight / watertight,
joints around shoot;
6 dry leaves / AW; max 4
7 keep , condition(s) / named condition(s) , constant;
8 allow time for shoot to acclimatise / AW;
9 shut screw clip;
10 keep ruler fixed and record position of air bubble on scale;
R ‘move bubble to end’ ideas
11 start timing and, measure / calculate, distance moved per unit time
/ AW;