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CHAD
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#1
Report Thread starter 17 years ago
#1
Hi everyone,
I am doing my biology coursework 4 gcse, and it is about the rate of a reaction with potato and a solution of hydrogen peroxide and water. I have done mine about the concentration of substrate (hydrogen peroxide). I was wondering if anyone could help me with the evaluation. I no basically wot u have 2 write but can anyone tell me wot else i can put as i want 2 get a really good grade cos i wanna do biology 4 as level.
Thanx 2 anyone who can help me!!!
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Tealer
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#2
Report 17 years ago
#2
Can you tell us how you did your original experiment then we can help you with ideas to make it better....

IE using measuring cylinder or a syringe to collect the gas instead of measuring height of bubbles given off.
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CHAD
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#3
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hi
below is my method, i thought that may help.

First of all I checked that all the safety measures I have previously mentioned were in place and I then started setting up the apparatus for my experiment. I secured the gas syringe to the right height on the clamp stand and I then proceeded to collect the rest of my apparatus so I could start carrying out my pilot investigation. Once I had got my other apparatus together I proceeded to fill a beaker three-quarters full of Hydrogen Peroxide (H2O2). I did this so I did not have to keep measuring out the amounts H2O2 that I needed straight into the measuring cylinder incase I over filled it and had to pour some back. This also meant that I did not need to keep going backwards and forwards to the bottle of H2O2 after each of my repeats was over. Next I mixed together my substrate solution which consisted, sometimes of water and Hydrogen Peroxide and I poured it into the conical flask that I was using in my experiments. I then chose a potato with as little number of holes in it as possible so I could get the largest number of discs possible out of the same potato – If I changed the potato, I could be changing other factors that may affect the outcome of my experiment. Next I cut the segments of potato into small discs 5mm long. I then checked that the gas syringe was placed on 0 and with the help of somebody else, I dropped the 20 discs of potato into my substrate solution, placed the bung on the conical flask and started the timer. I then recorded the amount of gas given off every 10 seconds for 2 minutes and in virtually all of my experiments, the enzyme and the substrate had not finished reacting at the end of the 2 minutes.

sorry its so long, but thank-you sssssssssssoooooooo much 4 offering 2 help!!!
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thefish_uk
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#4
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Point out anomalous results. Think if ideas for improving it, like using datalogging or something. This is really similar looking to my Biology coursework which is about concentration of NaCl solution and osmosis into... you guessed it - potatos.

I suppose concentration is the only numeric stuff in Biology and therefore the only stuff you can draw a graph for. Is yours about osmosis? We have to find the relative osmotic concentration (or something) of the contents of the cells in a potato.
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LH
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#5
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Say you could have used liver instead of potato as this also contains catalase and this could have given different results etc.
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thefish_uk
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#6
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catalase? what the hell is that... lol
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LH
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#7
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(Original post by thefish_uk)
catalase? what the hell is that... lol
An enzyme, found in potato and liver, central to this coursework...

C/w title =
The Affect of Substrate Concentration on the Rate of Reaction of the Enzyme Catalase
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thefish_uk
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#8
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oh yeah the "ase" bit always means enzyme
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nikk
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#9
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(Original post by thefish_uk)
oh yeah the "ase" bit always means enzyme
....good luck....
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NickNack
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#10
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tip - if you havent got any anomalouse points, make one. it gives you something to talk about and (depending on your teacher) youll get marks
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