Short Course in Protein Purification Using GFP

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Daniel Gonzalez
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Report 17 years ago
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I have been asked to announce the following,

The State University of New Jersey Rutgers (Campus at New Brunswick)

Center for Research and Education in Bioluminescence and Biotechnology Programs in
Biotechnology, Presents:

Protein Purification: Isolation, Analysis, and Characterization of GFP, A Five and One-Half Day
Hands-On Laboratory Course Using the remarkable Green-Fluorescent Protein (GFP), A Novel Marker For
Gene Expression, as the source material;

July 21-26, 2002 August 4-9, 2002 January 12-17, 2003 and March
16-21, 2003

More than 1100 scientists from around the world have strongly recommended this intensive course as
an opportunity to develop protein research and analytical skills in a retreat setting. Participants
work hard, identify

and solve problems in the lab and enjoy camaraderie and good food and beer with colleagues. An added
bonus of this course is practical laboratory exposure to the GFP, now a staple of life sciences
laboratories everywhere.

This five and one-half day laboratory course covers a wide variety of conventional methods for
protein isolation, purification, and characterization. The course format integrates hands-on
laboratory exercises with classroom lectures, demonstrations, study breaks, and short take-home
assignments.

A special feature of the course is that all laboratory work will be performed on the same starting
sample (native or recombinant Aequorea GFP), which will be purified from an exceedingly crude form
to near homogeneity as judged by high performance liquid chromatography (HPLC), SDS gel
electrophoresis, isoelectric focusing, and capillary zone electrophoresis. This feature provides a
continuity of purpose, integrating dozens of preparative and analytical protein techniques in a way
that few competing courses can match.

A problem-solving approach will be used throughout the course. Under the guidance of experienced lab
instructors, participants will work in groups of three to plan their own protocols, analyze data,
and interpret

results. A student-teacher ratio not greater than 8:1 will be maintained

and the faculty coordinators will be present throughout the course.

For further details you can reach us,

by E-mail at: [email protected] or [email protected] by phone at: (732) 932-9071 extension
225 or 216 by FAX at: (732) 932-3633

for a brochure and further information please visit the GFP purification

short course official Web site at: http://www.rci.rutgers.edu/~meton/protein.html
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