Turn on thread page Beta
    • Thread Starter
    Offline

    0
    ReputationRep:
    What would happen if I have 1ml of pure protein, Then I add some arbitrary amount of acid, for example 0.1ml 1M HCl?
    Offline

    16
    ReputationRep:
    Would it not cause the protein to be 'denatured' - which would however only really be affected if some kind of enzyme, perhaps pepsin, was present?

    By the way, I'm not a chemist so don't take my word for it, I am kind of testing myself here and awaiting other replies to correct me

    Have you actually looked anything up on this topic, digestion, for example?
    Offline

    19
    As above, you would disrupt the folding/bonding in the protein causing it to be denatured. The tolerance to acid is going to depend on the particular protein, though.
    • Thread Starter
    Offline

    0
    ReputationRep:
    Will there be any chemical changes to the protein?? I'm doing western blot on a protein of interest, and having it denatured isn't a big deal (or is it?)
    By chemical changes, I mean changes to he amino acid structures and stuff like that.
    Offline

    0
    ReputationRep:
    There are likely to be chemical changes to the protein. If the protein makes up an enzyme, then the active site would probably be denatured. (Most likely it'll changes shape in some way). Hence, the enzyme cannot attach itself on to the substrate molecule.

    If its just a "normal" protein, there are likely to be chemical changes. Some ion may displace the Hydrogen out of the acid.
    Offline

    0
    ReputationRep:
    Well, it would alter hydrogen bonds so it would denature anything biological that does not operate at the given pH.
    Offline

    14
    ReputationRep:
    It may alter the local and net charges on the molecule? :shrug:
 
 
 
Reply
Submit reply
Turn on thread page Beta
Updated: August 5, 2009
Poll
Could you cope without Wifi?

The Student Room, Get Revising and Marked by Teachers are trading names of The Student Room Group Ltd.

Register Number: 04666380 (England and Wales), VAT No. 806 8067 22 Registered Office: International House, Queens Road, Brighton, BN1 3XE

Write a reply...
Reply
Hide
Reputation gems: You get these gems as you gain rep from other members for making good contributions and giving helpful advice.