OCR Biology F215 Control, Genomes and Environment Fri 15 June 2012

OH! God. This book is CRAP! I think I've got a general idea of what it's rambling on about and if that's the case, It's explaining it in the WORST possible way.

The "labelled" nucleotides are the stop and start codons or primers, I think.

The not labelled ones are the free nucleotides which pair in complementary with the DNA fragment to create a separate strand of the DNA
- after it's been cut up by a restriction enzyme.

It stops the strand from becoming really long, because it doesn't need the rest, it just needs that particular section.

The electrophoresis simply shows if the DNA has been cut in that area or not by identifying fragments.

For example, if there is a gene that is 20 base pairs long, for a positive result, there will be a fragment that is 20 base pairs long showing up on the electrophoresis.

Sequencing a genome is the OCR way of saying how do we know what's in the DNA to identify if a person has a genetic disorder etc. I don't know the actual name of it but that's the meaning.

The Human Genome Project do it.

What's the mark scheme say?

Heyy can someone ask me questions on anything on unit 5 please and I will ask you some too!

Thanks!

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It is a technique where enzymes and substrate molecules collide forming an enzyme-substrate complex, so that enzymes are separate from the reaction mixture, avoiding inexpensive processes to seperate the two.
Correct me if im wrong okay your turn!

Explain the terms limiting factor and carrying capacity (2)

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Ooh oh sorry okay

explain the process of Polymerase Chain Reaction (PCR) (5)

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LOL ohh okay XD!

What kind of mutation is it when TTA becomes TCA? (1)

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LOL ohh okay XD!

What kind of mutation is it when TTA becomes TCA? (1)

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point mutation

Not specific enough to get the mark. Missense, nonsense and silent mutations are all caused by point mutations. The examiner wants to know the effect of the mutation on the protein.

http://cambridgeacademic.blogspot.co.uk/

it is specific enough look at the mark scheme !!!

I stand corrected http://www.thestudentroom.co.uk/images/smilies/smile.png although OCR can be quite fussy about exact words to get the marks so to be on the safe side I would say point mutation causing a missense change in the protein.

How much do you need to know about the sliding filament model of muscular contraction?

I'm seriously struggling with this Could someone explain this to me please and help me out?

Thats correct its only 1 mark, so I guess it is enough. Oh and another term for point mutation is substitution, just incase a questions asks you to talk about substitution, you'll know what it is !

Ask me ask me

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