ELISA Calibration Graph Analysis
Hi there,
I am currently attempting to analyse my calibration graph for an ELISA experiment that I have conducted. The graph is absorbance vs log concentration.
I am struggling to understand why the trendline must not go through 0. Why R2 value must be under 1 ? And what the line indicates - how would I know the concentration of the protein present from the trendline? And also why it is essential to minus Blank from the Absorbance? And how removing the high concentration result from the graph can effect it?
I would appreciate any help. Thank you!!
I am currently attempting to analyse my calibration graph for an ELISA experiment that I have conducted. The graph is absorbance vs log concentration.
I am struggling to understand why the trendline must not go through 0. Why R2 value must be under 1 ? And what the line indicates - how would I know the concentration of the protein present from the trendline? And also why it is essential to minus Blank from the Absorbance? And how removing the high concentration result from the graph can effect it?
I would appreciate any help. Thank you!!
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