Year 12s: what will be the first way you research your future options? >>

Ultracentrifugation

What is the difference between differential centrifugation and ultra centrifugation? What is involved in ultracentrifugation?

Reply 1

Kallisto

Entertainment Forum Helper

Original post by Isabelle.Kay21

What is the difference between differential centrifugation and ultra centrifugation? What is involved in ultracentrifugation?

What did you mean exactly when you wrote about 'involved'? the samples in them or the materials for operating the centrifuges?

Reply 2

Isabelle.Kay21

Original post by Kallisto

What did you mean exactly when you wrote about 'involved'? the samples in them or the materials for operating the centrifuges?

Like the process of ultracentrifugation and also how ultracentrifugation is linked to differential centrifugation

Reply 3

Kallisto

Entertainment Forum Helper

Original post by Isabelle.Kay21

Like the process of ultracentrifugation and also how ultracentrifugation is linked to differential centrifugation

So, I have searching for the differences in the Internet.

It seems that the process is the same, namely using the inertia of the samples to divide the substances. Substances with higher density are outside, the ones with lower density are in the middle. The differences is the higher acceleration and thus velocity which can be achieved by Ultracentrifugation. That leads to a higher number of revolutions for rotors (up to 500,000 revolutions per minute!)

Thanks to the higher velocity, even substances which are difficult to divide like microsomes (up to 100,000 g; g stands for the g-force value, the higher the greater the number of revolutions) can be segregated.

That is all what can I say to that.