Pyrazinamide effects on e-coli HELP

Hi, does anyone know why prazinamide doesnt effect e-coli? I understand pyrazinamide works by entering mycobacterium tuberculosis via diffusion to convert into pyrazinoic acid and then be excreted and reabsorbed. because the efflux pump is inefficient it causes cell damage. Does e-coli have like a more efficient pump where this doesnt happen? Help!

Reply 1

HarisMalik98

12

Yep, you're absolutely correct. M. tuberculosis is uniquely susceptible to pyrazinoic acid (POA) due to the inefficiency of its POA-associated efflux pump. Most other bacteria (including E. coli) do not have this issue since they can efficiently remove POA due to the increased activity of associated transporters. See here, where they generated E. coli mutants with deficient efflux pumps and showed susceptibility to POA: https://academic.oup.com/femsle/article/211/2/265/527825

Reply 2

BENgal232

OP

4

Original post by HarisMalik98

Yep, you're absolutely correct. M. tuberculosis is uniquely susceptible to pyrazinoic acid (POA) due to the inefficiency of its POA-associated efflux pump. Most other bacteria (including E. coli) do not have this issue since they can efficiently remove POA due to the increased activity of associated transporters. See here, where they generated E. coli mutants with deficient efflux pumps and showed susceptibility to POA: https://academic.oup.com/femsle/article/211/2/265/527825

Thankyou soo much! It did seem like the most logical answer, but I really wasn't too sure so thankyou for the confirmation! Ive just read the article and that is extremely fascinating and I will include it in my report!

Pyrazinamide effects on e-coli HELP

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