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Biology enzymes question

Can anyone help me with this A-Level Biology question?

Scientists investigated the effect of pH 8.4 and pH 7.5 on the activity of enzymes P and Q.
Figure 8, shows their results... This is not needed to answer the first question

Describe what the scientists should place in the control tubes in this investigation?
- same volume of each buffer solution
- same concentration of substrate
- same concentration of denatured enzyme

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