Difficult Biology Question...Hill reaction

How does Ammonium Hydroxide inhibit the decolourisation of DCPIP in the hill reaction?

If you cant Answer the top one. Here is another i need help with:

The chloroplast suspension contains lots singular chloroplast molecules right?

(edited 6 years ago)

Reply 1

SummerStrawberry

The Hill Reaction refers to the release of electrons in the light-dependent stage of photosynthesis, which reduce NADP to NADPH (you should know the importance of NADPH in the light-independent stage of photosynthesis). DCPIP has a very strong affinity for electrons, and so it - not NADP - is reduced, changing from blue to colourless.
Ammonium hydroxide is effectively a herbicide, which also has a very strong affinity for electrons, so accepts them over DCPIP, thereby preventing DCPIP from being reduced, inhibiting its decolourisation.

A chloroplast suspension contains lots of chloroplast organelles (not molecules).