F215 - Revision thread 13th June 2011

When sections of DNA are put into BACS to create clone libraries. Is that basically a slower version of PCR?

No its different, BAC is a larger version of genome sequencing. PCR is slightly different as your making many copies of the sample. BAC is what was used for the Human Genome Project. From what i have read and with teacher input i think we just need to know the outline of BAC without much detail as we will need to know genome sequencing in more detail.

Genome sequencing is for up to about 100,000 bases.
BAC is for 3900,000,000 bases (the human genome).

Reply 441

Jacinta_kid

Have you all finished the course? We finished Genes and Genomes, but haven't started Plant Responses and we've finished, so we have to teach ourselves! Any advice please?
And I'm revising muscle contractions, I need a B so lots of work!!

Reply 442

slacker07906

OP

16

Right I got hold of my teacher and she categorically told me that we don't need to know about BAC's. What we need to know for sequencing is the automated termination method. Thanks god o_o I was having mini- mycardial infarctions!

Reply 443

twelve

13

Original post by Jacinta_kid

Have you all finished the course? We finished Genes and Genomes, but haven't started Plant Responses and we've finished, so we have to teach ourselves! Any advice please?
And I'm revising muscle contractions, I need a B so lots of work!!

Plant responses is a fairly easy topic - the textbook does cover it pretty much exactly. Its mostly evaluating experimental data, so as long as you vaguely understand the usual functions of each plant hormone, you should be fine :smile:

And the only thing thats different about plant hormones and human hormones is that plant hormones can affect different tissues in different ways, and sometimes can inhibit eachother and sometimes stimulate greater production of another hormone. They behave pretty unpredictably really!

Original post by slacker07906

Right I got hold of my teacher and she categorically told me that we don't need to know about BAC's. What we need to know for sequencing is the automated termination method. Thanks god o_o I was having mini- mycardial infarctions!

Thank god for that - I was getting scared seeing everyone on here going on about BACs, knowing it had only been given as an example by my teacher...
So relieved now :smile:

Also, who's doing the random repping on this thread... Seems like people have been repped for really unrepworthy questions haha

Reply 444

Waqar Y

11

BAC is one method of sequencing a genome, chain termination is another method.

BAC is basically just inserting the DNA into E.coli and letting them multiply, from there you use restriction enzymes and begin the chain termination method.

Reply 445

merrmerr

I am totally freaking out over this exam!! i need 120/150 in this AND 72/90 in my f214 resit to get an A !
Does this sound unrealistic to achieve? What ums does everyone need??

Also, can someone actually clarify what allopatric anf sympatric speciation is?? Thanks!

Reply 446

Dunn0

1

Can someone please help explain what exactly we need to know about artifical selection of wheat?
Thanks

Reply 447

lola1993

11

Also, can someone actually clarify what allopatric anf sympatric speciation is?? Thanks!

Think allopatric is where they inhabit different geographical areas and sympatric is where they inhabit the same area.

Reply 448

greenford

do you think that punnet square could come up.... genetics.... what are the chances?

Reply 449

twelve

13

Original post by greenford

do you think that punnet square could come up.... genetics.... what are the chances?

Definitely - its a REALLY big topic, and apparently it hasn't come up much in the past.

Reply 450

duskflower

6

Anyone care to sum-up Immobilized enzymes? The different types? I've just spent the last six hours revising and want to learn something else with the least possible work involved :rolleyes:

Reply 451

periwinkle304

8

Original post by slacker07906

.

Original post by tesha_al

.

thanks for your help :smile:

Reply 452

periwinkle304

8

Original post by lola1993

Think allopatric is where they inhabit different geographical areas and sympatric is where they inhabit the same area.

do we have to know about different types of speciation?? :s-smilie:

Reply 453

Wor Carroll

Original post by merrmerr

I am totally freaking out over this exam!! i need 120/150 in this AND 72/90 in my f214 resit to get an A !
Does this sound unrealistic to achieve? What ums does everyone need??

Also, can someone actually clarify what allopatric anf sympatric speciation is?? Thanks!

How do you work out what you need in f215 because I'd like to know mine?
BTW I don't think that is too unrealistic but it'll require quite a bit of revision and understanding.

Allopatric speciation is geographical isolation. It involves a combination of genetic drift and natural selection.
Sympatric speciation is when it's not geographically isolated, so this occurs in the same area. It can be a result of polyploidy.

Reply 454

sportycricketer

Original post by Wor Carroll

How do you work out what you need in f215 because I'd like to know mine?
BTW I don't think that is too unrealistic but it'll require quite a bit of revision and understanding.

Allopatric speciation is geographical isolation. It involves a combination of genetic drift and natural selection.
Sympatric speciation is when it's not geographically isolated, so this occurs in the same area. It can be a result of polyploidy.

If you want me to work it out, PM me your module UMS and what you got in your practical /40 this year and I can tell you :smile:

Reply 455

dildobaggins

ok guys i need some help, i am looking over genome sequencing and have come to find out that there are two ways of sequencing: the BAC to BAC method and then the shotgun sequencing method. Can someone please outline what are the steps to the sequencing method we need to know as its driving me nuts. thanks

Reply 456

MoMatrix

Does anyone just try to memorise certain topics without having real understanding?
I feel I am hitting a dead end with some stuff, I am just trying to parrot memorise it.................

Reply 457

fortunecookie

17

Original post by Mobs25

No its different, BAC is a larger version of genome sequencing. PCR is slightly different as your making many copies of the sample. BAC is what was used for the Human Genome Project. From what i have read and with teacher input i think we just need to know the outline of BAC without much detail as we will need to know genome sequencing in more detail.

Genome sequencing is for up to about 100,000 bases.
BAC is for 3900,000,000 bases (the human genome).

Ahh I see, that makes sense. I guess you'd have to do PCR a LOT in order to replicate it, so a lot of effort.

Yeah, was just trying to get my head around it. Because in the 'outline DNA sequencing' it had the use of BAC's. So when is PCR used? Replicating DNA when searching for genetic diseases? I hate all this stuff!

Reply 458

fortunecookie

17

Original post by Wor Carroll

How do you work out what you need in f215 because I'd like to know mine?
BTW I don't think that is too unrealistic but it'll require quite a bit of revision and understanding.

Allopatric speciation is geographical isolation. It involves a combination of genetic drift and natural selection.
Sympatric speciation is when it's not geographically isolated, so this occurs in the same area. It can be a result of polyploidy.

This has stuff for all of my subjects, but when you come to the biology one type in your UMS marks and on the right hand side it will tell you what you need in F215.