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Electrophoresis results

If you carried out reverse transcription PCR, then subjected the results to agarose gel electrophoresis is possible that MRNA could give a result in the bands produced ?

Reply 1

King Boo

It depends on how much mRNA was in the sample that you reverse transcribed. Remember in RT PCR you take mRNA and convert it to a strand of cDNA which is then copied to another strand to form a complimentary strand to complete the double strand.

You would expect to see some mRNA on the gel though. As the ethidium bromide would treat it as DNA all the same so it makes sense it would show.